Role of Pulmonary Phagocytes in Host Defense against Group B Streptococci inPreterm versus Term Rabbit Lung

Abstract

Intrapulmonary clearance of group B streptococci (GBS) occurred in term rabbits 4 and 8 h after infection; GBS growth was evident in preterm rabbits at 8 h. Bronchoalveolar lavage revealed 17-fold higher numbers of pulmonary aveolar macrophages (PAM) in term versus pre-term animals immediately after infection, whereas polymorphonuclear leukocyte (PMNL) recruitment was 13-fold greater in preterm than term rabbits at 8 h. Anti-CD18 monoclonal antibody R15.7 did not reduce PMNL influx or GBS killing in term animals. R15.7 failed to inhibit PMNL influx but augmented GBS growth in preterm animals. R15.7 significantly impaired GBS phagocytosisby preterm and term PMNL in vitro but had no effecton ingestion of GBS by preterm and term PAM. Thus, GBS infection initiates PMNL recruitment into lungs of preterm rabbits by CD18-independent mechanisms, but phagocytosis of GBS by PMNL is largely CD18-dependent. The poorer outcome ofGBS pneumonia in preterm versus term newborns may result from low levelsof PAM, thereby mandating recruitment of PMNL as a second phagocytic defense.