Murine hematopoietic blast colony-forming cells and their progeny have distinctive membrane marker profiles
- 10 November 2009
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 106 (45), 19102-19107
- https://doi.org/10.1073/pnas.0910354106
Abstract
Two distinct bone marrow-derived blast colony-forming cells can generate colonies of lineage-restricted progenitor cells in agar cultures of murine bone marrow. Both cell types selectively had a Kit(+) ScaI(+) phenotype distinguishing them from most lineage-restricted progenitor cells. Multicentric blast colony-forming cells stimulated by stem cell factor plus interleukin-6 (IL-6) (BL-CFC-S) were separable from most dispersed blast colony-forming cells stimulated by Flt3 ligand and IL-6 (BL-CFC-F) using CD34 and Flt3R probes. Multicentric BL-CFC-S cofractionated with colony-forming units, spleen (CFU-S) supporting the possibility that the 2 cells may be identical. The colony populations generated by BL-CFC-S were similar in their phenotype and proliferative capacity to progenitor cells in whole bone marrow but the progeny of BL-CFC-F were skewed with an abnormally high proportion of Kit(-) Flt3R(+) cells whose clonogenic cells tended to generate only macrophage progeny. Both blast colony populations had a high percentage of GR1(+) and Mac1(+) cells but BL-CFC-F colonies also contained a significant population of B220(+) and IL-7R(+) cells relevant to the superior ability of BL-CFC-F colony cells to generate B lymphocytes and the known dependency of this process on Flt3 ligand and IL-7. The commitment events and phenotypic changes during the generation of differing progenitor cells in blast colonies can now be clonally analyzed in a convenient in vitro culture system.Keywords
This publication has 12 references indexed in Scilit:
- Reductive isolation from bone marrow and blood implicates common lymphoid progenitors as the major source of thymopoiesisBlood, 2009
- Two distinct types of murine blast colony-forming cells are multipotential hematopoietic precursorsProceedings of the National Academy of Sciences of the United States of America, 2008
- Identification of Lin–Sca1+kit+CD34+Flt3– short-term hematopoietic stem cells capable of rapidly reconstituting and rescuing myeloablated transplant recipientsBlood, 2005
- Key Role of flt3 Ligand in Regulation of the Common Lymphoid Progenitor but Not in Maintenance of the Hematopoietic Stem Cell PoolImmunity, 2002
- Stem cells, cancer, and cancer stem cellsNature, 2001
- Stem and Progenitor Cells: Origins, Phenotypes, Lineage Commitments, and TransdifferentiationsAnnual Review of Cell and Developmental Biology, 2001
- Mice lacking flt3 ligand have deficient hematopoiesis affecting hematopoietic progenitor cells, dendritic cells, and natural killer cells.2000
- A clonogenic common myeloid progenitor that gives rise to all myeloid lineagesNature, 2000
- The Hemopoietic Colony-stimulating FactorsPublished by Cambridge University Press (CUP) ,1995
- A Direct Measurement of the Radiation Sensitivity of Normal Mouse Bone Marrow CellsRadiation Research, 1961