Effect of Substrate Concentration on Carbon Isotope Fractionation during Acetoclastic Methanogenesis by Methanosarcina barkeri and M. acetivorans and in Rice Field Soil
- 1 May 2009
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 75 (9), 2605-2612
- https://doi.org/10.1128/aem.02680-08
Abstract
Methanosarcina is the only acetate-consuming genus of methanogenic archaea other than Methanosaeta and thus is important in methanogenic environments for the formation of the greenhouse gases methane and carbon dioxide. However, little is known about isotopic discrimination during acetoclastic CH 4 production. Therefore, we studied two species of the Methanosarcinaceae family, Methanosarcina barkeri and Methanosarcina acetivorans , and a methanogenic rice field soil amended with acetate. The values of the isotope enrichment factor (ε) associated with consumption of total acetate (ε ac ), consumption of acetate-methyl (ε ac-methyl ) and production of CH 4 (ε CH4 ) were an ε ac of −30.5‰, an ε ac-methyl of −25.6‰, and an ε CH4 of −27.4‰ for M. barkeri and an ε ac of −35.3‰, an ε ac-methyl of −24.8‰, and an ε CH4 of −23.8‰ for M. acetivorans . Terminal restriction fragment length polymorphism of archaeal 16S rRNA genes indicated that acetoclastic methanogenic populations in rice field soil were dominated by Methanosarcina spp. Isotope fractionation determined during acetoclastic methanogenesis in rice field soil resulted in an ε ac of −18.7‰, an ε ac-methyl of −16.9‰, and an ε CH4 of −20.8‰. However, in rice field soil as well as in the pure cultures, values of ε ac and ε ac-methyl decreased as acetate concentrations decreased, eventually approaching zero. Thus, isotope fractionation of acetate carbon was apparently affected by substrate concentration. The ε values determined in pure cultures were consistent with those in rice field soil if the concentration of acetate was taken into account.Keywords
This publication has 37 references indexed in Scilit:
- Methanocella paludicola gen. nov., sp. nov., a methane-producing archaeon, the first isolate of the lineage 'Rice Cluster I', and proposal of the new archaeal order Methanocellales ord. nov.International Journal of Systematic and Evolutionary Microbiology, 2008
- Identification of Acetate-Assimilating Microorganisms under Methanogenic Conditions in Anoxic Rice Field Soil by Comparative Stable Isotope Probing of RNAApplied and Environmental Microbiology, 2007
- The Methanosarcina barkeri Genome: Comparative Analysis with Methanosarcina acetivorans and Methanosarcina mazei Reveals Extensive Rearrangement within Methanosarcinal GenomesJournal of Bacteriology, 2006
- Carbon Isotope Fractionation during Acetoclastic Methanogenesis by Methanosaeta concilii in Culture and a Lake SedimentApplied and Environmental Microbiology, 2006
- Determination of isotope fractionation factors and quantification of carbon flow by stable carbon isotope signatures in a methanogenic rice root model systemGeobiology, 2006
- Metabolism of position-labelled glucose in anoxic methanogenic paddy soil and lake sedimentFEMS Microbiology Letters, 1991
- Temporal change of gas metabolism by hydrogen-syntrophic methanogenic bacterial associations in anoxic paddy soilFEMS Microbiology Letters, 1989
- Biogeochemical aspects of atmospheric methaneGlobal Biogeochemical Cycles, 1988
- Methane formation from acetyl phosphate in cell extracts of Methanosarcina barkeri Dependence of the reaction on coenzyme AFEBS Letters, 1988
- Stable carbon isotope ratios and the existence of a gas phase in the evolution of carbonate ground watersGeochimica et Cosmochimica Acta, 1974