Enzyme-digested Fucoidan Extracts Derived from Seaweed Mozuku of Cladosiphon novae-caledoniae kylin Inhibit Invasion and Angiogenesis of Tumor Cells
- 1 January 2005
- journal article
- research article
- Published by Springer Science and Business Media LLC in Cytotechnology
- Vol. 47 (1-3), 117-126
- https://doi.org/10.1007/s10616-005-3761-8
Abstract
Fucoidan is a uniquely-structured sulfated polysaccharide found in the cell walls of several types of brown seaweed that has recently, especially as enzyme-digested fucoidan extract, attracted a lot attention due to its anti-tumor potential. In this study, we evaluated the effects of enzyme-digested fucoidan extracts prepared from seaweed Mozuku of Cladosiphon novae-caledoniae kylin on in vitro invasion and angiogenesis abilities of human tumor cells. First, we evaluated the effect of the fucoidan extracts on oxidative stress of tumor cells, and demonstrated that intracellular H2O2 level and released H2O2 from tumor cells were both greatly repressed upon the treatment with the fucoidan extracts, suggesting that fucoidan extracts ameliorate oxidative stress of tumor cells. Next, we tested for the effects of fucoidan extracts on invasion ability of human fibrosarcoma HT1080 cells, showing that fucoidan extracts significantly inhibit their invasion, possibly via suppressing matrix metalloproteinases (MMPs) MMP-2/9 activities. Further, we investigated the effects of the fucoidan extracts on angiogenesis of human uterine carcinoma HeLa cells, and found that fucoidan extracts suppressed expression and secretion of an angiogenesis factor vascular endothelial growth factor (VEGF), resulting in suppressed vascular tubules formation of tumor cells. The results taken together clarified that enzyme-digested fucoidan extracts from Cladosiphon novae-caledoniae kylin possess inhibitory effects on invasion and angiogenesis of tumor cells. These effects might, at least partially, be elicited by the antioxidative potential of enzyme digested fucoidan extracts.Keywords
This publication has 48 references indexed in Scilit:
- Low Molecular Weight Fucoidan and Heparin Enhance the Basic Fibroblast Growth Factor-Induced Tube Formation of Endothelial Cells through Heparan Sulfate-Dependent α6 OverexpressionMolecular Pharmacology, 2003
- Mechanical Stretch Enhances mRNA Expression and Proenzyme Release of Matrix Metalloproteinase-2 (MMP-2) via NAD(P)H Oxidase–Derived Reactive Oxygen SpeciesCirculation Research, 2003
- Hydrogen Peroxide Formation and Actin Filament Reorganization by Cdc42 Are Essential for Ethanol-induced in Vitro AngiogenesisJournal of Biological Chemistry, 2003
- Matrix metalloproteinase-9 expression in post-hypoxic human brain capillary endothelial cells: H2O2 as a trigger and NF-κB as a signal transducerThrombosis and Haemostasis, 2003
- Ultraviolet B and H2O2 Are Potent Inducers of Vascular Endothelial Growth Factor Expression in Cultured KeratinocytesJournal of Biological Chemistry, 1996
- Vascular Permeability Factor (Vascular Endothelial Growth Factor) Expression and Angiogenesis in Cervical NeoplasiaJNCI Journal of the National Cancer Institute, 1995
- Inhibitory Effect of Oversulfated Fucoidan on Invasion through Reconstituted Basement Membrane by Murine Lewis Lung CarcinomaJapanese Journal of Cancer Research, 1994
- Vascular endothelial growth factorTrends in Cardiovascular Medicine, 1993
- Inhibitory effects of fucoxanthin, a natural carotenoid, on N-ethyl-N′-nitro-N-nitrosoguanidine-induced mouse duodenal carcinogenesisCancer Letters, 1993
- Isolation of highly purified "Fucoidan" from Eisenia bicyclis and its anticoagulant and antitumor activities.Agricultural and Biological Chemistry, 1980