RNA pre-amplification enables large-scale RT-qPCR gene-expression studies on limiting sample amounts
Open Access
- 25 November 2009
- journal article
- research article
- Published by Springer Science and Business Media LLC in BMC Research Notes
- Vol. 2 (1), 1-9
- https://doi.org/10.1186/1756-0500-2-235
Abstract
No abstract availableKeywords
This publication has 26 references indexed in Scilit:
- Reliability of real-time reverse-transcription PCR in clinical diagnostics: gold standard or substandard?Expert Review of Molecular Diagnostics, 2009
- Linear mRNA Amplification from as Little as 5 ng Total RNA for Global gene Expression AnalysisBioTechniques, 2004
- Evaluation of techniques using amplified nucleic acid probes for gene expression profilingBiomolecular Engineering, 2003
- SMART amplification maintains representation of relative gene expression: quantitative validation by real time PCR and application to studies of alcoholic liver disease in primatesJournal of Biochemical and Biophysical Methods, 2003
- Representation is faithfully preserved in global cDNA amplified exponentially from sub-picogram quantities of mRNANature Biotechnology, 2002
- Gene quantification using real-time quantitative PCRExperimental Hematology, 2002
- Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assaysJournal of Molecular Endocrinology, 2000
- Detection of rare mRNAs via quantitative RT-PCRTrends in Genetics, 1992
- Analysis of gene expression in single live neurons.Proceedings of the National Academy of Sciences, 1992
- Amplified RNA synthesized from limited quantities of heterogeneous cDNA.Proceedings of the National Academy of Sciences, 1990