Biosynthesis of Infectious Tobacco Mosaic Virus Ribonucleic Acid in a Cell-Free Medium
- 5 October 1962
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 138 (3536), 46-48
- https://doi.org/10.1126/science.138.3536.46
Abstract
When cell-free preparations containing large molecules and subcellular bodies extracted from tobacco-leaf cells infected with tobacco mosaic virus were incubated with MgCl2 and the four ribonucleoside-5'-triphosphates, the level of infectivity increased about threefold in 30 minutes. The mechanism synthesizing the new infectivity was believed to be located within isolated nuclei. Ultrasonic rupture of the nuclear membranes appeared to increase the rate of synthesis.Keywords
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