Low molecular weight RNAs transcribed in vitro by RNA polymerase III from Alu-type dispersed repeats in Chinese hamster DNA are also found in vivo.

Abstract

An Alu-type dispersed repeat previously identified in a cloned fragment of Chinese hamster DNA serves as a template for cell-free transcription of discrete low MW RNA by RNA polymerase III [RNA nucleotidyltransferase (DNA-directed)]. A class of analogous RNA was isolated from growing Chinese hamster cells by hybridization of total low MW nuclear RNA to the cloned DNA fragment from which cell-free transcription occurs. Two-dimensional analysis of RNase digestion products of these RNA suggests that they are transcribed from multiple members of the Alu-type dispersed repeat family.