Quantitative regional determination of morphometric indices of the total and perfused capillary network in the rat brain.
- 1 October 1982
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in Circulation Research
- Vol. 51 (4), 494-503
- https://doi.org/10.1161/01.res.51.4.494
Abstract
A new method was developed to simultaneously and regionally determined various morphometric indices of the perfused and total capillary network in rat brain on a quantitative basis. A high molecular weight dextran, labeled with fluorescein isothiocyanate (FITC), was injected into the femoral vein of a barbiturate anesthetized rat. After 20 s, the animal was decapitated and the head frozen in liquid nitrogen. The anterior cortex, hypothalamus, thalamus, lenticulate nuclei, substantia nigra, hippocampus, posterior cortex, cerebellum, pons and medulla were isolated and mounted in a microtome cryostat. Sections, 2 .mu.m thick, were photographed with a fluorescent microscope to detect the perfused capillaries. The sections then were stained for alkaline phosphatase to visualize the total capillary network. Standard morphometric techniques were employed to determine the total and perfused volume, surface area, length, diameter and number per cubic millimeter from the photographs. The method was validated in brain, muscle and omentum to show that the technique did not alter the capillary network, that both the total and perfused capillary network were visualized, and that there was little tissue shrinkage. The study found no significant differences in the total capillary network on a regional basis in rat brain in comparisons of any of the above indices in the 10 large brain areas. The perfused capillary network was not different on a regional basis. Within the cortex, both total and perfused indices were greater in grey than white matter. The perfused capillary network ranged from 50.1-56.9% of total for the various indices determined. The percentage of the capillary network perfused increased significantly in a series of asphyxiated rats. Diameter of the capillaries in the control series averaged 6.05 .+-. 1.09 .mu.m (mean .+-. SD) and was not significantly greater in the portion of the bed which was perfused.This publication has 21 references indexed in Scilit:
- Effect of papaverine on regional cerebral blood flow and small vessel blood contentEuropean Journal of Pharmacology, 1980
- Cerebral Regional Oxygen Consumption and Supply in Anesthetized CatScience, 1980
- ALKALINE PHOSPHATASE ACTIVITY IN CEREBRAL BLOOD VESSELS OF SPONTANEOUSLY HYPERTENSIVE RAT : The Second Report of Enzyme Histochemical Studies of SHR BrainJapanese Circulation Journal, 1979
- Microvascular supply in relation to fiber metabolic type in mixed skeletal muscles of rabbitsMicrovascular Research, 1978
- Quantitative relations between the number of ramifications and the length of capillaries in the neocortex of rat during postnatal developmentBrain Structure and Function, 1973
- Brain blood flow in the conscious and anesthetized ratAmerican Journal of Physiology-Legacy Content, 1973
- GEFÄSSANORDNUNG UND KAPILLARDICHTE IM GEHIRN DES KANINCHENSCells Tissues Organs, 1965
- Beiträge zur Histochemie der Blutgefäßendothelien und der CapillargrundhäutchenVirchows Archiv, 1958
- THE ARCHITECTURE OF THE CEREBRAL CAPILLARY BEDBiological Reviews, 1945
- On the relative vascularity of various parts of the central nervous system of the albino ratJournal of Comparative Neurology, 1920