Transformability of Haemophilus influenzae
- 1 November 1962
- journal article
- research article
- Published by Microbiology Society in Journal of General Microbiology
- Vol. 29 (3), 537-549
- https://doi.org/10.1099/00221287-29-3-537
Abstract
A method is described for the preparation of highly competent (transformable) bacteria of Haemophilus influenzae. After three successive treatments), i.e. aerobic growth, anaerobic incubation and incubation in saline containing a few per cent broth, transformation frequencies close to 5% have been obtained. Competent bacteria absorb DNA very fast. Untransformable ones lack this ability. Absorption is inhibited by dinitrophenol or arsenate; it is not inhibited by chloramphenicol. This suggests the participation of an enzyme system. Development of competency is blocked by dinitrophenol, arsenate or chloramphenicol. It is strongly dependent upon temperature. It is not influenced by the absorption of one or two DNA molecules per bacterium.Keywords
This publication has 3 references indexed in Scilit:
- Studies on the Mechanism of Radiation Inactivation of Microorganisms: VIII. Inactivation of Intracellular Multiply Marked Haemophilus Influenzae Deoxyribonucleic Acid by High-Voltage ElectronsRadiation Research, 1961
- Studies on Transformations of Hemophilus influenzae The Journal of general physiology, 1961
- STUDIES ON THE CHEMICAL NATURE OF THE SUBSTANCE INDUCING TRANSFORMATION OF PNEUMOCOCCAL TYPESThe Journal of Experimental Medicine, 1944