Genetic Analysis of Simian Virus 40 IV. Inhibited Transformation of Balb/3T3 Cells by a Temperature-Sensitive Early Mutant
- 1 June 1972
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 9 (6), 969-972
- https://doi.org/10.1128/jvi.9.6.969-972.1972
Abstract
The temperature-sensitive early mutant, ts*101, was characterized during productive infection in monkey cells, and the results are presented in an accompanying paper. This paper demonstrates that although 101 mutant virions adsorb normally to confluent Balb/3T3 mouse cells at both permissive (33 C) and restrictive (38.5 C) temperatures, T antigen synthesis and transformation, abortive and stable, are inhibited at both temperatures (host-range inhibition). T antigen synthesis is temperature sensitive, whereas abortive and stable transformation are not. Clones of 101-transformed Balb/3T3 cells were isolated, and virus was rescued from all clones at both permissive and restrictive temperatures. The rescued virus was as temperature sensitive as the original transforming 101 virions.Keywords
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