SurA assists the folding of Escherichia coli outer membrane proteins
- 1 March 1996
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 178 (6), 1770-1773
- https://doi.org/10.1128/jb.178.6.1770-1773.1996
Abstract
Many proteins require enzymatic assistance in order to achieve a functional conformation. One rate-limiting step in protein folding is the cis-trans isomerization of prolyl residues, a reaction catalyzed by prolyl isomerases. SurA, a periplasmic protein of Escherichia coli, has sequence similarity with the prolyl isomerase parvulin. We tested whether SurA was involved in folding periplasmic and outer membrane proteins by using trypsin sensitivity as an assay for protein conformation. We determined that the efficient folding of three outer membrane proteins (OmpA, OmpF, and LamB) requires SurA in vivo, while the folding of four periplasmic proteins was independent of SurA. We conclude that SurA assists in the folding of certain secreted proteins.Keywords
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