Cyclic Nucleotide Concentrations in Tissue and Perfusate of Isolated Rat Lung
- 1 January 1984
- journal article
- Published by Informa UK Limited in Experimental Lung Research
- Vol. 7 (1), 67-76
- https://doi.org/10.3109/01902148409087909
Abstract
Cyclic nucleotide content of lung tissue is altered by anesthesia, ventilatory pattern, and pharmacologic manipulation (e.g., isoproterenol). In addition the lung releases cyclic nucleotides into its circulation, but little is known about factors that might alter this release. We isolated and perfused rat lungs (IPL) to determine.- 1) if cyclic nucleotides are released into the perfusate in the control state; and 2) if their release changes after alteration of the ventilatory pattern or the addition of isoproterenol. We demonstrated that the rat IPL releases both cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) into the perfusate. Isoproterenol has no effect on cGMP release but increases cAMP release dramatically. Perfusate cAMP is not affected by ventilatory pattern, but perfusate cGMP is higher during high-pressure ventilation than it is in nonventilated or normally ventilated lungs.Keywords
This publication has 11 references indexed in Scilit:
- Isoproterenol and aminophylline reduce lung capillary filtration during high permeabilityJournal of Applied Physiology, 1979
- Cyclic AMP and Cyclic GMP in Canine Peripheral Lung: RegulationIn Vivo1–4American Review of Respiratory Disease, 1978
- Lung tissue guanosine 3',5'-monophosphate: effects of ventilation and anesthesiaJournal of Applied Physiology, 1978
- Cyclic nucleotide activity in gastrointestinal tissues and fluidsAnalytical Biochemistry, 1977
- Effects of decapitation, ether and pentobarbital on guanosine 3′,5′-phosphate and adenosine 3′,5′-phosphate levels in rat tissuesBiochimica et Biophysica Acta (BBA) - General Subjects, 1974
- Rat lung metabolism: glucose utilization by isolated perfused lungs and tissue slicesAmerican Journal of Physiology-Legacy Content, 1974
- Sources of Cyclic Nucleotides in PlasmaJCI Insight, 1974
- Regulation by β-adrenergic receptor and muscarinic cholinergic receptor activation of intracellular cyclic AMP and cyclic GMP levels in rat lung slicesBiochemical and Biophysical Research Communications, 1973
- Cyclic 3′,5′-adenosine monophosphate response in the rabbit lung—Adult properties and developmentBiochemical Pharmacology, 1972
- EXTRACELLULAR CYCLIC NUCLEOTIDES*Annals of the New York Academy of Sciences, 1971