The complete genome sequence of a novel hypovirus infecting Bipolaris oryzae
- 13 February 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Archiv für die gesamte Virusforschung
- Vol. 165 (4), 1027-1031
- https://doi.org/10.1007/s00705-020-04556-z
Abstract
Hypoviruses are positive-sense single-stranded RNA mycovirus that infect filamentous fungi. However, hypoviruses have not been reported in Bipolaris oryzae, an important phytopathogenic fungus in water bamboo and rice. Here, we report the characterization of a novel hypovirus, tentatively named “Bipolaris oryzae hypovirus 1” (BoHV1), isolated from strain ES35 of B. oryzae infecting water bamboo. The complete genome of BoHV1 consists of 13,596 nucleotides and a poly(A) tail at the 3’ end. BoHV1 has single open reading frame (ORF) and encodes a putative polyprotein (4,218 amino acids) containing four potential conserved domains for a papain-like protease, a protein of unknown function (DUF3525), RNA-dependent RNA polymerase (RdRp), and helicase. Phylogenetic analysis of the polyprotein, RdRp, and helicase domains suggested that BoHV1 belongs to the genus Hypovirus within the family Hypoviridae. This is the first report of the presence of a hypovirus in the phytopathogenic fungus B. oryzae.Keywords
Funding Information
- Special Funds for “Double First-Class” Construction in Hubei Province
- Open Fund of Hubei Key Laboratory of Biological Resources Protection and Utilization (PKLHB1909)
- Hubei Technical Innovation Project (2017ABA152)
- National Key R&D Program of China (2017YFD0201103)
This publication has 16 references indexed in Scilit:
- ICTV Virus Taxonomy Profile: HypoviridaeJournal of General Virology, 2018
- Molecular characterization of a novel hypovirus from the plant pathogenic fungus Fusarium graminearumVirology, 2015
- 50-plus years of fungal virusesVirology, 2015
- Synonymy of two species of Bipolaris from aquatic crops of PoaceaeMycotaxon, 2015
- The genusBipolarisStudies in Mycology, 2014
- Molecular characterization of two positive-strand RNA viruses co-infecting a hypovirulent strain of Sclerotinia sclerotiorumVirology, 2014
- Characterisation of a novel hypovirus from Sclerotinia sclerotiorum potentially representing a new genus within the HypoviridaeVirology, 2014
- A review on crop losses, epidemiology and disease management of rice brown spot to identify research priorities and knowledge gapsEuropean Journal of Plant Pathology, 2013
- Molecular characterization of a new hypovirus infecting a phytopathogenic fungus, Valsa ceratospermaVirus Research, 2012
- Hypovirulence: Mycoviruses at the fungal–plant interfaceNature Reviews Microbiology, 2005