Subclinical Chlamydial Infection of the Female Mouse Genital Tract Generates a Potent Protective Immune Response: Implications for Development of Live Attenuated Chlamydial Vaccine Strains
- 1 January 2000
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 68 (1), 192-196
- https://doi.org/10.1128/iai.68.1.192-196.2000
Abstract
Chlamydia trachomatis is a major cause of sexually transmitted disease (STD) for which a vaccine is needed. CD4 + T-helper type 1 (Th1) cell-mediated immunity is an important component of protective immunity against murine chlamydial genital infection. Conventional vaccine approaches have not proven effective in eliciting chlamydial-specific CD4 Th1 immunity at the genital mucosa. Thus, it is possible that the development of a highly efficacious vaccine against genital infection will depend on the generation of a live attenuated C. trachomatis vaccine. Attenuated strains of C. trachomatis do not exist, so their potential utility as vaccines cannot be tested in animal models of infection. We have developed a surrogate model to study the effect of chlamydial attenuation on infection and immunity of the female genital tract by treating mice with a subchlamydiacidal concentration of oxytetracycline following vaginal infection. Compared to untreated control mice, antibiotic-treated mice shed significantly fewer infectious organisms (3 log 10 ) from the cervico-vagina, produced a minimal inflammatory response in urogenital tissue, and did not experience infection-related sequelae. Antibiotic-treated mice generated levels of chlamydia-specific antibody and cell-mediated immunity equivalent to those of control mice. Importantly, antibiotic-treated mice were found to be as immune as control untreated mice when rechallenged vaginally. These findings demonstrate that subclinical chlamydial infection of the murine female genital tract is sufficient to stimulate a potent protective immune response. They also present indirect evidence supporting the possible use of live attenuated chlamydial organisms in the development of vaccines against chlamydial STDs.Keywords
This publication has 52 references indexed in Scilit:
- Genome Sequence of an Obligate Intracellular Pathogen of Humans: Chlamydia trachomatisScience, 1998
- Studies in Knockout Mice Reveal that Anti‐Chlamydial Protection Requires TH1 Cells Producing IFN‐γ: Is this True for Humans?Scandinavian Journal of Immunology, 1997
- Chlamydia trachomatis-Associated Ectopic Pregnancy: Serologic and Histologic CorrelatesThe Journal of Infectious Diseases, 1992
- TH1 and TH2 Cells: Different Patterns of Lymphokine Secretion Lead to Different Functional PropertiesAnnual Review of Immunology, 1989
- Etiology and Outcome of Acute Pelvic Inflammatory DiseaseThe Journal of Infectious Diseases, 1988
- Chlamydia trachomatis infections in the United States. What are they costing us?Published by American Medical Association (AMA) ,1987
- Vaccination with temperature-sensitive mutant of Chlamydia psittaci against enzootic abortion of ewesPublished by Wiley ,1984
- Chlamydia trachomatis-Induced Salpingitis in MiceThe Journal of Infectious Diseases, 1983
- Chlamydial InfectionsNew England Journal of Medicine, 1978