High Efficiency Latency and Activation of Herpes Simplex Virus in Human Cells
- 17 September 1982
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 217 (4565), 1145-1146
- https://doi.org/10.1126/science.6180477
Abstract
Herpes simplex virus (HSV) exists in humans in a latent form that can be activated. To characterize the molecular basis of the cell-virus interactions and to analyze the state of the latent HSV genome, an in vitro model system was established. In this system a large fraction of the latently infected cells contain an HSV genome that can be activated. Cell survival was reduced minimally after repression of high multiplicity HSV type 1 (HSV-1) infection of human fibroblast cells with (E)-5-(2-bromovinyl)-2'-deoxyuridine in combination with human leukocyte interferon (IFN-alpha). A minimum of 1 to 3 percent of the surviving cells contained an HSV genome that could be activated either by human cytomegalovirus superinfection or reduction in incubation temperature.Keywords
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