Identification of Apo-A1 as a biomarker for early diagnosis of bladder transitional cell carcinoma
Open Access
- 17 April 2011
- journal article
- Published by Springer Science and Business Media LLC in Proteome Science
- Vol. 9 (1), 21
- https://doi.org/10.1186/1477-5956-9-21
Abstract
Background: Bladder transitional cell carcinoma (BTCC) is the fourth most frequent neoplasia in men, clinically characterized by high recurrent rates and poor prognosis. Availability of urinary tumor biomarkers represents a convenient alternative for early detection and disease surveillance because of its direct contact with the tumor and sample accessibility. Results: We tested urine samples from healthy volunteers and patients with low malignant or aggressive BTCC to identify potential biomarkers for early detection of BTCC by two-dimensional electrophoresis (2-DE) coupled with mass spectrometry (MS) and bioinformatics analysis. We observed increased expression of five proteins, including fibrinogen (Fb), lactate dehydrogenase B (LDHB), apolipoprotein-A1 (Apo-A1), clusterin (CLU) and haptoglobin (Hp), which were increased in urine samples of patients with low malignant or aggressive bladder cancer. Further analysis of urine samples of aggressive BTCC showed significant increase in Apo-A1 expression compared to low malignant BTCC. Apo-A1 level was measured quantitatively using enzyme-linked immunosorbent assay (ELISA) and was suggested to provide diagnostic utility to distinguish patients with bladder cancer from controls at 18.22 ng/ml, and distinguish patients with low malignant BTCC from patients with aggressive BTCC in two-tie grading system at 29.86 ng/ml respectively. Further validation assay showed that Apo-A1 could be used as a biomarker to diagnosis BTCC with a sensitivity and specificity of 91.6% and 85.7% respectively, and classify BTCC in two-tie grading system with a sensitivity and specificity of 83.7% and 89.7% respectively. Conclusion: Taken together, our findings suggest Apo-A1 could be a potential biomarker related with early diagnosis and classification in two-tie grading system for bladder cancer.Keywords
This publication has 41 references indexed in Scilit:
- Discovery of Novel Bladder Cancer Biomarkers by Comparative Urine Proteomics Using iTRAQ TechnologyJournal of Proteome Research, 2010
- Morphological diagnosis of urothelial neoplasmsJournal of Clinical Pathology, 2007
- Elevation of serum l-lactate dehydrogenase B correlated with the clinical stage of lung cancerLung Cancer, 2006
- Institutional variability in the accuracy of urinary cytology for predicting recurrence of transitional cell carcinoma of the bladderBJU International, 2006
- Proteome analysis of hepatocellular carcinoma by laser capture microdissectionProteomics, 2006
- Differential gene expression profiling in aggressive bladder transitional cell carcinoma compared to the adjacent microscopically normal urothelium by microdissection-SMART cDNA PCR-SSHCancer Biology & Therapy, 2006
- Stage-dependent increase of orosomucoid and zinc-alpha2-glycoprotein in urinary bladder cancerProteomics, 2005
- Bladder CancerEuropean Urology, 2001
- CLINICAL USEFULNESS OF THE NOVEL MARKER BLCA-4 FOR THE DETECTION OF BLADDER CANCERJournal of Urology, 2000
- Bladder caner from a perspective of 40 yearsJournal of Cellular Biochemistry, 1992