Characterization of viremia at different stages of varicella-zoster virus infection
- 1 September 1998
- journal article
- research article
- Published by Wiley in Journal of Medical Virology
Abstract
Varicella-zoster virus (VZV) viremia at different stages of infection was characterized. Different approaches were used, polymerase chain reaction (PCR), isothermal transcription based nucleic acid amplification (NASBA), and immunofluorescence to describe and quantitate viral infection of peripheral blood mononuclear cells (PBMC). In patients with acute varicella 200 to 5,000 copies of the viral genome in every 150,000 PBMC were found with quantitative competitive PCR (QCPCR). With NASBA, viral transcriptional activity was detected in these cells. RNA transcribed from the immediate early gene IE 63 as well as from the late gene 68 were found, indicating a productive infection. Glycoprotein gE specific immunofluorescence visualized by confocal laser scanning microscopy revealed that only 1 in 10,000 to 100,000 PBMC was infected. T and B lymphocytes as well as monocytes expressed viral protein on their surface. Similar results were obtained with PBMC from immunocompetent zoster patients. In some cases a transient viremia was found shortly after the onset of rash, although the viral load seemed to be lower than in patients with varicella. Examination of blood samples from 16 persons with postherpetic neuralgia (PHN) signs of viral replication in PBMC were not detected. In conclusion, the data suggest that VZV viremia is a frequent event in patients with varicella and zoster, but not in those with postherpetic neuralgia. Moreover, the results indicated that subclinical reactivations occur both in immunocompromised and immunocompetent individuals. J. Med. Virol. 56:91–98, 1998.Keywords
This publication has 20 references indexed in Scilit:
- Postherpetic Neuralgia — Pathogenesis, Treatment, and PreventionNew England Journal of Medicine, 1996
- Distribution of varicella‐zoster virus gpI and gpII and corresponding genome sequences in the skinJournal of Medical Virology, 1995
- Nucleic acid sequence-based amplification (NASBA) for the identification of mycobacteriaJournal of General Microbiology, 1993
- Detection of HIV-1 Distribution in Different Blood Fractions by Two Nucleic Acid Amplification AssaysAIDS Research and Human Retroviruses, 1993
- Diagnosis of acute and latent varicella-zoster virus infections using the polymerase chain reactionJournal of Medical Virology, 1991
- Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA PolymeraseScience, 1988
- The Complete DNA Sequence of Varicella-Zoster VirusJournal of General Virology, 1986
- Clinical and Subclinical Reactivations of Varicella-Zoster Virus in Immunocompromised PatientsThe Journal of Infectious Diseases, 1986
- Viremia is present in incubation period in nonimmunocompromised children with varicellaThe Journal of Pediatrics, 1985
- A viremic phase for herpes zoster in children with cancerThe Journal of Pediatrics, 1977