Mitochondrial proteomics on human fibroblasts for identification of metabolic imbalance and cellular stress
Open Access
- 1 January 2009
- journal article
- Published by Springer Science and Business Media LLC in Proteome Science
- Vol. 7 (1), 20
- https://doi.org/10.1186/1477-5956-7-20
Abstract
Mitochondrial proteins are central to various metabolic activities and are key regulators of apoptosis. Disturbance of mitochondrial proteins is therefore often associated with disease. Large scale protein data are required to capture the mitochondrial protein levels and mass spectrometry based proteomics is suitable for generating such data. To study the relative quantities of mitochondrial proteins in cells from cultivated human skin fibroblasts we applied a proteomic method based on nanoLC-MS/MS analysis of iTRAQ-labeled peptides.Keywords
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