Steady growth cone currents revealed by a novel circularly vibrating probe: A possible mechanism underlying neurite growth

Abstract

The rate and direction of neurite growth have been shown in a number of studies to be determined by the distribution of adhesive sites on the growth cone. Recent evidence showing that the application of extrinsic electric fields can redistribute membrane molecules and alter both the rate and direction of neurite growth have raised the question whether endogenous electric fields might be produced by steady currents in growth cones. To investigate this question, we have devised a novel circularly vibrating microprobe capable of measuring current densities in the range of 5 nA/cm² (near the theoretical limit of sensitivity), with a spatial resolution of 2 μm. The design of this device and the development of a novel algorithm for computing current vectors on-line is described. Using this probe we have found that cultured goldfish retinal ganglion cell growth cones generate steady inward currents goldfish retinal ganglion cell growth cones generate steady inward currents at their tips. The measured currents, in the range of 10–100 nA/cm², appear to flow into the filopodia at their tips and back outward near the junctures of the filopodia and the growth cone. The currents appear to be produced only during active growth. Ion substitution experiments support the conclusion that the majority of this current is carried by Ca²⁺ ions, which we postulate flow through a population of activated voltage-sensitive Ca²⁺ channels located on the filopodial tips. Calculation of the transmembrane current density (4 × 10⁻⁶ nA/cm²) leads to an estimate of channel density (10 channels/μm²) in close agreement with the measured density of Ca²⁺ channels in other systems. The assumption that calcium channel proteins are conveyed to nerve terminals by active transport, whereas sodium channel proteins are conveyed passively by a slower somatofugal diffusion process [Strichartz et al, 1984], would explain why developing neurons tend to display Ca²⁺-sensitive electrogenesis at their growing tips, and Na⁺-sensitive action potentials later in development. In order to gain some insight into the possible role of these steady growth currents, we estimated the membrane depolarization and axial voltage gradient they produce. It is likely that the currents produce sufficient membrane depolarization (≅ 4 mV) to cause autogenous activation of ion channel permeabilities. Similarly, the axial voltage gradient (≅4 mV/cm) would be expected to move intracytoplasmic vesicles by electrophoresis at a rate (20–40 pm/hr) very close to that at which the filopodia are observed to grow. These considerations lead us to propose that growth cone currents set up both a Ca²⁺ gradient and an electric field that play significant roles in growth. These are likely to include both the transport and exocytotic fusion of vesicles into growing membrane, as well as the alignment of molecules involved in contraction and adhesion. A steady calcium current would also be associated with the spontaneous release of neurotransmitter from the growth cone, as recently observed [Young and Poo, 19831] and a mechanism is proposed whereby this release might serve to autofocus receptor molecules at sites of contact with target cells. In order to determine whether the extracellular electric fields produced by the measured growth cone currents might be of sufficient magnitude to affect the distribution of surface membrane molecules by external lateral electrophoresis, we applied focal electric fields to growth cones with a micropipette. The observed field strengths necessary to affect filopodial orientation (70-350 mV/cm) were several orders of magnitude larger than those produced by growth cone currents. We conclude that the physiological effects of growth cone currents are confined to the growth cone, wher'e they may play a significant role in the fusion of new membrane, the release of transmitter, and the transport and alignment of membrane molecules.