Influence of carriers on the development and localization of anti‐2,4,6‐trinitrophenyl (TNP) antibody‐forming cells in the murine spleen: II. Suppressed antibody response to TNP‐Ficoll after elimination of marginal zone cells
- 1 January 1986
- journal article
- research article
- Published by Wiley in European Journal of Immunology
- Vol. 16 (5), 492-497
- https://doi.org/10.1002/eji.1830160505
Abstract
The macrophages in the marginal zones in mice spleens were eliminated by i.v. injection of dichloromethylene diphosphonate encapsulated in liposomes. After immunization with a thymus-dependent (trinitrophenylated keyhole limpet hemocyanin; TNP-KLH) or thymus-independent type 1 (TNP-lipopolysaccharide) antigen, no differences in antibody responses in treated and untreated mice were observed. However, immunization with a thymus-independent type 2 (TI-2) antigen (TNP-Ficoll) resulted in a strong decrease of the antibody response in macrophage-depleted animals. Anti-TNP serum levels dropped 10-fold and the number of antibody-forming cells in the spleen 30-60-fold. These results confirm the special role of the marginal zone macrophages in the processing of TI-2 antigens, as earlier suggested in splenectomy studies.This publication has 28 references indexed in Scilit:
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