Neurotransmitters decrease the calcium conductance activated by depolarization of embryonic chick sensory neurones.

Abstract

Several neurotransmitters including noradrenaline [norepinephrine] (NA), GABA and serotonin [5-hydroxytryptamine] (5-HT), and certain peptides decrease the duration of the Na+-Ca2+ action potential recorded in cell bodies of embryonic chick dorsal root ganglion neurons maintained in cell culture. To determine if these agents decreased action potential duration by affecting Ca2+ channels (inward current) or K+ channels (outward current) membrane currents were recorded in voltage-clamped sensory neuron somata. Depolarization produced a prominent inward Na+ current and a smaller and slower inward Ca2+ current (ICa). The inactivation of ICa was not simply dependent on membrane potential but apparently required prior entry of Ca2+. Two components of outward current, voltage-activated and Ca2+-activated, were evident in most cells. The effect of NA, and also of GABA and 5-HT, resulted from a direct effect on ICa because NA decreased the TTX[tetrodotoxin]-resistant tail current recorded at EK and the inward current recorded in the presence of 125 mM-TEA and TTX (in which Na+ and K+ currents were blocked). The decrease in ICa is most likely due to an effect on the number of available Ca2+ channels and/or the single Ca2+ channel conductance rather than to a shift in either the kinetics of channel activation or the Ca2+ equilibrium potential. No effect of the several transmitters on the voltage-dependent Na+ and K+ currents was observed. Implications of ICa modulation for the phenomenon of presynaptic inhibition are discussed.