Map-Based Cloning of a Gene Controlling Omega-3 Fatty Acid Desaturation in Arabidopsis
- 20 November 1992
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 258 (5086), 1353-1355
- https://doi.org/10.1126/science.1455229
Abstract
A gene from the flowering plant Arabidopsis thaliana that encodes an omega-3 desaturase was cloned on the basis of the genetic map position of a mutation affecting membrane and storage lipid fatty acid composition. Yeast artificial chromosomes covering the genetic locus were identified and used to probe a seed complementary DNA library. A complementary DNA clone for the desaturase was identified and introduced into roots of both wild-type and mutant plants by Ti plasmid-mediated transformation. Transgenic tissues of both mutant and wild-type plants had significantly increased amounts of the fatty acid produced by this desaturase.Keywords
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