Validation of an Improved Reversed-Phase High-Performance Liquid Chromatography Assay With Reductive Electrochemical Detection for the Determination of Artemisinin Derivatives in Man
- 1 February 1998
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in Therapeutic Drug Monitoring
- Vol. 20 (1), 109-116
- https://doi.org/10.1097/00007691-199802000-00020
Abstract
For the determination of artemisinin (ART) and analogs, a reversed-phase high-performance liquid chromatography method using reductive electrochemical detection (ED) was set up with some important modifications as compared to previously published assays. A different technique of deoxygenating resulted in a factor 2-3 lower background current. A Spectroflow 400 liquid chromatograph in combination with a Triathlon autoinjector coupled to a Decade electrochemical detector was used. The detector was operated in the reductive mode as a closed system under chromatography grade helium to exclude any access of oxygen. The Decade has a glassy carbon electrode and a reference Ag/AgCl electrode. Infrequent electropolishing was required implicating a very stable system. By increasing acetonitril or lowering the pH of the mobile phase, the various derivatives could be determined in the same chromatogram. The assay was validated using artemether (ATM) and dihydroartemisinin (DHA) as test substances. In the concentration range seen in people after usual doses (5 to 220 ng/ml), the assay performs with adequate accuracy and precision. The interassay and intraassay precision are 0.98) was achieved. The method was applied in a study in which artemether was administered orally to healthy white subjects. We consider high-performance liquid chromatography with electrochemical detection an accurate and precise method for quantitative determination of artemisinin derivatives in pharmacokinetic studies.Keywords
This publication has 13 references indexed in Scilit:
- Determination of artemether and its metabolite, dihydroartemisinin, in plasma by high-performance liquid chromatography and electrochemical detection in the reductive modeJournal of Chromatography B: Biomedical Sciences and Applications, 1997
- Determination of artemether and dihydroartemisinin in blood plasma by high-performance liquid chromatography for application in clinical pharmacological studiesJournal of Chromatography B: Biomedical Sciences and Applications, 1995
- Packed-column supercritical fluid chromatography of artemisinin (qinghaosu) with electron-capture detectionJournal of Chromatography B: Biomedical Sciences and Applications, 1995
- Chemiluminescent detection of artemisinin novel endoperoxide analysis using luminol without hydrogen peroxideJournal of Chromatography A, 1995
- Clinical pharmacokinetics and pharmacodynamics of artemisinin and derivativesTransactions of the Royal Society of Tropical Medicine and Hygiene, 1994
- Measurement of artemisinin and its derivatives in biological fluidsTransactions of the Royal Society of Tropical Medicine and Hygiene, 1994
- An overview of the clinical use of artemisinin and its derivatives in the treatment of falciparum malaria in Viet NamTransactions of the Royal Society of Tropical Medicine and Hygiene, 1994
- Artemisinin: current statusTransactions of the Royal Society of Tropical Medicine and Hygiene, 1994
- Determination of the Antimalarial Arteether and its Deethylated Metabolite Dihydroartemisinin in Plasma by High-Performance Liquid Chromatography with Reductive Electrochemical DetectionJournal of Pharmaceutical Sciences, 1991
- Analysis of artesunic acid and dihydroqinghaosu in blood by high-performance liquid chromatography with reductive electrochemical detectionJournal of Chromatography B: Biomedical Sciences and Applications, 1987