Molecularly imprinted polymer solid-phase extraction coupled to square wave voltammetry at carbon fibre microelectrodes for the determination of fenbendazole in beef liver
- 13 March 2007
- journal article
- research article
- Published by Springer Science and Business Media LLC in Analytical and Bioanalytical Chemistry
- Vol. 388 (1), 227-234
- https://doi.org/10.1007/s00216-007-1233-z
Abstract
A molecularly imprinted polymer was developed and used for solid-phase extraction (MISPE) of the antihelmintic fenbendazole in beef liver samples. Detection of the analyte was accomplished using square wave voltammetry (SWV) at a cylindrical carbon fibre microelectrode (CFME). A mixture of MeOH/HAc (9:1) was employed both as eluent in the MISPE system and as working medium for electrochemical detection of fenbendazole. The limit of detection was 1.9 × 10−7 mol L−1 (57 μg L−1), which was appropriate for the determination of fenbendazole at the maximum residue level permitted by the European Commission (500 μg kg−1 in liver). Given that the SW voltammetric analysis could not be directly performed in the sample extract as a consequence of interference from some sample components, a sample clean-up with a MIP for selectively retaining fenbendazole was performed. The MIP was synthesized using a 1:8:22 template/methacrylic acid/ethylene glycol dimethacrylate ratio. A Britton–Robinson Buffer of pH 9.0 was selected for retaining fenbendazole in the MIP cartridges, and an eluent volume of 5.0 mL at a flow rate of 2.0 mL min−1 was chosen in the elution step. Cross-reactivity with the MIP was observed for other benzimidazoles. The synthesized MIP exhibited a good selectivity for benzimidazoles with respect to other veterinary drugs. The applicability of the MISPE-SWV method was tested with beef liver samples, spiked with fenbendazole at 5,000 and 500 μg kg−1. Results obtained for ten different liver samples yielded mean recoveries of (95 ± 12)% and (96 ± 11)% for the upper and lower concentration level, respectively.This publication has 27 references indexed in Scilit:
- Solid-phase molecularly imprinted on-line preconcentration and voltammetric determination of sulfamethazine in milkAnalytica Chimica Acta, 2005
- Comparison of milk residue profiles after oral and subcutaneous administration of benzimidazole anthelmintics to dairy cowsAnalytica Chimica Acta, 2005
- Multi-residue method for the determination of benzimidazoles in bovine liverAnalytica Chimica Acta, 2005
- Retention mechanism of analytes in the solid-phase extraction process using molecularly imprinted polymersJournal of Chromatography B, 2004
- Clean-up of triazines in vegetable extracts by molecularly-imprinted solid-phase extraction using a propazine-imprinted polymerAnalytical and Bioanalytical Chemistry, 2003
- Molecularly imprinted polymers for on-line clean up and preconcentration of chloramphenicol prior to its voltammetric determinationAnalytical and Bioanalytical Chemistry, 2003
- Capillary electrophoresis with (R)-(−)-N-(3,5-dinitrobenzoyl)-α-phenylglycine as chiral selector for separation of albendazole sulfoxide enantiomers and their analysis in human plasmaJournal of Pharmaceutical and Biomedical Analysis, 2002
- On-Line Solid-Phase Extraction of Triazine Herbicides Using a Molecularly Imprinted Polymer for Selective Sample EnrichmentAnalytical Chemistry, 1999
- Direct Drug Determination by Selective Sample Enrichment on an Imprinted PolymerAnalytical Chemistry, 1994
- Determination of clenbuterol in bovine urine by enzyme immunoassay following concentration and clean-up by immunoaffinity chromatographyAnalytica Chimica Acta, 1993