Site‐specific expression of CD11b and SIRPα (CD172a) on dendritic cells: implications for their migration patterns in the gut immune system
Open Access
- 22 April 2005
- journal article
- research article
- Published by Wiley in European Journal of Immunology
- Vol. 35 (5), 1418-1427
- https://doi.org/10.1002/eji.200425726
Abstract
Dendritic cells (DC) in the intestinal tract play a major role in directing the mucosal immune system towards tolerance or immunity. We analyzed whether different mucosal DC subsets in pigs have specific functions, localizations, or migration patterns in vivo. Therefore, we collected physiologically migrating DC by pseudo‐afferent cannulation of the intestinal duct in eight Göttingen minipigs. Lymph DC were phenotypically and functionally characterized and compared to DC found on histological sections of porcine small intestine and mesenteric lymph nodes (MLN). Four different DC subpopulations were detected. Lamina propria (LP) DC were mainly CD11b+ signal regulatory protein α (SIRPα)+, DC in Peyer's patches were mainly CD11b–/SIRPα+ in subepithelial domes and CD11b–/SIRPα– in interfollicular regions, whereas MLN DC were largely CD11b+/SIRPα–. Of these four subsets, only the CD11b+/SIRPα+ DC and the CD11b+/SIRPα– DC were present in lymph. This suggests that DC migration to MLN largely originates from the LP. Lymph DC expressed high levels of MHC class II and costimulatory molecules and had a low capacity for FITC‐dextran uptake, indicating a mature phenotype. However, lymph DC did not induce PBMC proliferation in MLR, and migration was not significantly influenced by mucosal antigen application.Keywords
This publication has 34 references indexed in Scilit:
- Enrichment for a CD26hi SIRP− subset in lymph dendritic cells from the upper aero-digestive tractJournal of Leukocyte Biology, 2004
- Porcine peripheral blood dendritic cells and natural interferon‐producing cellsImmunology, 2003
- Differences in cytokine synthesis by the sub-populations of dendritic cells from afferent lymphImmunology, 2003
- Proliferating intestinal γ/δ T cells recirculate rapidly and are a major source of the γ/δ T cell pool in the peripheral bloodEuropean Journal of Immunology, 2003
- Anatomical basis of tolerance and immunity to intestinal antigensNature Reviews Immunology, 2003
- Immature, semi-mature and fully mature dendritic cells: which signals induce tolerance or immunity?Trends in Immunology, 2002
- Interferon-alpha-producing cells are localized in gut-associated lymphoid tissues in transmissible gastroenteritis virus (TGEV) infected pigletsVeterinary Research, 2001
- Professional and non‐professional antigen‐presenting cells in the porcine small intestineImmunology, 2000
- Localization of Distinct Peyer's Patch Dendritic Cell Subsets and Their Recruitment by Chemokines Macrophage Inflammatory Protein (Mip)-3α, Mip-3β, and Secondary Lymphoid Organ ChemokineThe Journal of Experimental Medicine, 2000
- Distinct populations of dendritic cells are present in the subepithelial dome and T cell regions of the murine Peyer's patch.The Journal of Experimental Medicine, 1996