The Use of Solid Media for Detection of Enzyme Production by Fungi

Abstract
Solid media were described on which the production of the extracellular enzymes amylase, lipase, DNA- and RNAase, pectinase, protease, urease and chitinase were detected. The media were tested with 7 plant pathogenic (Fusarium oxysporum, Helminthosporium sp., Phytophthora spp., Ustilago maydis), 6 saprophytic (Aspergillus spp., Cladosporium sp., Mucor sp., Penicillium sp., Trichoderma viride) fungi, and a sample of leaf compost. Antibiotics were examined for their ability to suppress bacterial growth in the fungal media. The effects of antibiotics and pH on fungal growth and extracellular enzyme production were examined. The solid media described could be useful for evaluating individual fungi and for rapid screening of genetic variants for the presence or absence of enzyme production, as well as for ecological studies and possible chemotaxonomic differentiation.