Regulation of immature cartilage growth by IGF-I, TGF-β1, BMP-7, and PDGF-AB: role of metabolic balance between fixed charge and collagen network
- 29 August 2007
- journal article
- research article
- Published by Springer Science and Business Media LLC in Biomechanics and Modeling in Mechanobiology
- Vol. 7 (4), 263-276
- https://doi.org/10.1007/s10237-007-0096-8
Abstract
Cartilage growth may involve alterations in the balance between the swelling tendency of proteoglycans and the restraining function of the collagen network. Growth factors, including IGF-I, TGF-β1, BMP-7, and PDGF-AB, regulate chondrocyte metabolism and, consequently, may regulate cartilage growth. Immature bovine articular cartilage explants from the superficial and middle zones were incubated for 13 days in basal medium or medium supplemented with serum, IGF-I, TGF-β1, BMP-7, or PDGF-AB. Variations in tissue size, accumulation of proteoglycan and collagen, and tensile properties were assessed. The inclusion of serum, IGF-I, or BMP-7 resulted in expansive tissue growth, stimulation of proteoglycan deposition but not of collagen, and a diminution of tensile integrity. The regulation of cartilage metabolism by TGF-β1 resulted in tissue homeostasis, with maintenance of size, composition, and function. Incubation in basal medium or with PDGF-AB resulted in small volumetric and compositional changes, but a marked decrease in tensile integrity. These results demonstrate that the phenotype of cartilage growth, and the associated balance between proteoglycan content and integrity of the collagen network, is regulated differentially by certain growth factors.Keywords
This publication has 99 references indexed in Scilit:
- Tissue Growth and RemodelingAnnual Review of Biomedical Engineering, 2004
- Growth of Immature Articular Cartilage in Vitro: Correlated Variation in Tensile Biomechanical and Collagen Network PropertiesTissue Engineering, 2003
- Osteogenic protein-1 promotes the synthesis and retention of extracellular matrix within bovine articular cartilage and chondrocyte culturesOsteoarthritis and Cartilage, 2000
- Age- and injury-dependent concentrations of transforming growth factor-β1 and proteoglycan fragments in rabbit knee joint fluidOsteoarthritis and Cartilage, 1998
- Type II collagen and TGF-βs in developing and aging porcine mandibular condylar cartilage: immunohistochemical studiesCell and tissue research, 1997
- BMP-7 is an inducer of nephrogenesis, and is also required for eye development and skeletal patterning.Genes & Development, 1995
- A Triphasic Theory for the Swelling and Deformation Behaviors of Articular CartilageJournal of Biomechanical Engineering, 1991
- Growth and growth pressure of mandibular condylar and some primary cartilages of the rat in vitroAmerican Journal of Orthodontics and Dentofacial Orthopedics, 1986
- Chemical composition and swelling of normal and osteoarthrotic femoral head cartilage. II. Swelling.Annals Of The Rheumatic Diseases, 1977
- The resorption of bone collagen in tissue cultureBiochemical and Biophysical Research Communications, 1963