Detection of a plant protein analogous to the yeast spliceosomal protein, PRP8
- 22 February 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 318 (1), 4-6
- https://doi.org/10.1016/0014-5793(93)81315-q
Abstract
We have investigated whether a splieeosomal protein analogous to the yeast protein, PRP8, was present in higher plants. A protein with a molecular weight > 200 kDa was detected in Western blots of tobacco (Nicotiana tabacum L.) nuclear extracts with affinity-purified antibodies, raised against four different β-galactosidase-PRP8 fusion proteins. The > 200 kDa protein was also immunoprecipitated by antibodies against the snRNA-specific trimethylguanosine cap structure and was, therefore, snRNP-associated. The presence of this protein in plants, in addition to yeast, Drosophila and humans, and the conservation of large size and epitopes highlights the importance of PRP8 in pre-mRNA splicing.Keywords
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