Frozen Section Technique to Evaluate Early Burn Wound Biopsy

Abstract

The importance of early diagnosis treatment of burn wound infection has prompted many efforts to use frozen section technique for processing burn wound biopsies, most of which have been unsuccessful. A frozen section technique which facilitates quick, reliable evaluation of biopsies was developed, and has been used in the evaluation of 169 biopsies over a period of 18 months. The frozen section technique takes 30 minutes compared with 4 hours for the rapid section method. Comparison of diagnoses made using both methods for each of the 169 biopsies produced a 96% coincidence. Each discrepant diagnosis was corrected by the rapid technique approximately 3 1/2 hours after the frozen section diagnosis. The frozen section technique is a generally accurate and rapid means of assessing the microbial status of a burn wound and diagnosing invasive infection. Permanent sections produced by rapid section technique should always be examined to confirm the frozen section diagnosis.