GAS CHROMATOGRAPHIC METHOD FOR THE SEPARATION AND ESTIMATION OF AMINO ACID DERIVATIVES
- 1 March 1965
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry
- Vol. 43 (3), 309-315
- https://doi.org/10.1139/o65-039
Abstract
It has been possible to prepare the N-trifluoroacetyl methyl esters of the 19 amino acids commonly found in proteins and to separate them by gas chromatography on two types of column, Carbowax 1540 and Carbowax 20M. There is a constant relation between the amount of each amino acid and the size of the recorded peak. This indicates either that the conversion to the derivative is quantitative or that a constant proportion of each amino acid is converted to its N-trifluoroacetyl methyl ester.Keywords
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