FTIR Spectroscopic Study of Biogenic Mn-Oxide Formation byPseudomonas putidaGB-1
- 1 July 2005
- journal article
- research article
- Published by Informa UK Limited in Geomicrobiology Journal
- Vol. 22 (5), 207-218
- https://doi.org/10.1080/01490450590947724
Abstract
Biomineralization in heterogeneous aqueous systems results from a complex association between pre-existing surfaces, bacterial cells, extracellular biomacromolecules, and neoformed precipitates. Fourier transform infrared (FTIR) spectroscopy was used in several complementary sample introduction modes (attenuated total reflectance [ATR], diffuse reflectance [DRIFT], and transmission) to investigate the processes of cell adhesion, biofilm growth, and biological Mn-oxidation by Pseudomonas putida strain GB-1. Distinct differences in the adhesive properties of GB-1 were observed upon Mn oxidation. No adhesion to the ZnSe crystal surface was observed for planktonic GB-1 cells coated with biogenic MnO x , whereas cell adhesion was extensive and a GB-1 biofilm was readily grown on ZnSe, CdTe, and Ge crystals prior to Mn-oxidation. IR peak intensity ratios reveal changes in biomolecular (carbohydrate, phosphate, and protein) composition during biologically catalyzed Mn-oxidation. In situ monitoring via ATR-FTIR of an active GB-1 biofilm and DRIFT data revealed an increase in extracellular protein (amide I and II) during Mn(II) oxidation, whereas transmission mode measurements suggest an overall increase in carbohydrate and phosphate moieties. The FTIR spectrum of biogenic Mn oxide comprises Mn-O stretching vibrations characteristic of various known Mn oxides (e.g., “acid” birnessite, romanechite, todorokite), but it is not identical to known synthetic solids, possibly because of solid-phase incorporation of biomolecular constituents. The results suggest that, when biogenic MnO x accumulates on the surfaces of planktonic cells, adhesion of the bacteria to other negatively charged surfaces is hindered via blocking of surficial proteins.Keywords
This publication has 56 references indexed in Scilit:
- Microbial Polysaccharides Template Assembly of Nanocrystal FibersScience, 2004
- Molecular characterization of cyanobacterial silicification using synchrotron infrared micro-spectroscopyGeochimica et Cosmochimica Acta, 2004
- Characterization of Mn oxides in cemented streambed crusts from Pinal Creek, Arizona, U.S.A., and in hot-spring deposits from Yuno-Taki Falls, Hokkaido, JapanAmerican Mineralogist: Journal of Earth and Planetary Materials, 2002
- ATR–FTIR Spectroscopic Investigation on Phosphate Adsorption Mechanisms at the Ferrihydrite–Water InterfaceJournal of Colloid and Interface Science, 2001
- Enzymatic Manganese(II) Oxidation by a Marine α-ProteobacteriumApplied and Environmental Microbiology, 2001
- Mechanisms of adhesion of Paenibacillus polymyxa onto hematite, corundum and quartzInternational Journal of Mineral Processing, 2001
- Bacterial Mn2+Oxidizing Systems and Multicopper Oxidases: An Overview of Mechanisms and FunctionsGeomicrobiology Journal, 2000
- Structure of synthetic monoclinic Na-rich birnessite and hexagonal birnessite; I, Results from X-ray diffraction and selected-area electron diffractionAmerican Mineralogist: Journal of Earth and Planetary Materials, 1997
- Changes in the Secondary Structure of Adsorbed IgG and F(ab‘)2 Studied by FTIR SpectroscopyLangmuir, 1996
- Manganese oxidation by Leptothrix discophoraJournal of Bacteriology, 1987