THE ISOLATION AND ASSAY OF HYPERTENSIN FROM BLOOD

Open Access

1 March 1952

journal article
Published by Rockefeller University Press in The Journal of Experimental Medicine

Vol. 95 (3), 241-246
https://doi.org/10.1084/jem.95.3.241

Abstract

A method has been described for isolation and assay of hypertensin from the blood of dogs. An ethanol filtrate of blood drawn from the femoral artery is prepared and concentrated by evaporation. The hypertensin is extracted into n-butanol from which it is adsorbed into alumina, and subsequently eluted with dilute ethanol. The eluate is then evaporated to dryness, dissolved in water, and assayed by intravenous injection into rats.

Keywords

ANGIOTONIN/DETERMINATION

This publication has 2 references indexed in Scilit:

The Isolation of Hypertensin from the Circulating Blood of Normal Dogs with Experimental Renal Hypertension by Dialysis in an Artificial Kidney
Cell Metabolism, 1951
The Isolation of Hypertensin from the Circulating Blood of Dogs by Dialysis in an Artificial Kidney
Cell Metabolism, 1950

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