Extraction, Cleanup, and Quantitative Determination of Aflatoxins B1 and M1 in Beef Liver

Abstract

A method for the determination of aflatoxin Bx in eggs was applicable for aflatoxin B1 in liver, but ineffective for aflatoxin Mx in liver because of poor recovery of added aflatoxin and interferences in thin layer chromatography. The method was modified by the addition of citric acid to the extracting solvent and ammonium sulfate to the extract solution for removing protein. The elution system for silica gel column cleanup was also changed by substituting methanol for acetone, and adding a step for confirmation of aflatoxin M₁ identity. The method has been used successfully for survey and research on aflatoxin residues in animal tissues.