Redistribution of Foscan® from plasma proteins to model membranes
- 26 June 2006
- journal article
- research article
- Published by Springer Science and Business Media LLC
- Vol. 5 (8), 770-777
- https://doi.org/10.1039/b603840a
Abstract
Photodynamic therapy is a comparatively novel modality of tumours treatment that includes simultaneous action of photosensitizers, light and oxygen. Photosensitizer redistribution between plasma proteins and biomembranes define photosensitizers interaction with cells, their intracellular localization and kinetics of sensitizers accumulation in the tumour. Present study investigates the kinetics of Foscan® release from plasma proteins to model membranes using fluorescence resonance energy transfer (FRET) from label, covalently bound to protein, to sensitizer. We have demonstrated very slow kinetics of Foscan® release from protein complexes with rate constants of (1.7 ± 0.1) × 10−3 s−1 for albumin and (1.6 ± 0.3) × 10−4 s−1 for high-density lipoproteins (HDL). Foscan® redistributes by both collision and diffusion-mediated transfer from complexes with HDL, with bimolecular rate constant kout = (8.8 ± 1.4) × 10−2 M−1 s−1. Thermodynamic considerations proposed that sensitizer release from HDL into the aqueous medium is unfavourable and collision mechanism appeared to be a preferred mode of transfer in biological environment. Slow rates of Foscan® redistribution from plasma proteins should be considered while planning dosimetry protocol of Foscan®-PDT.Keywords
This publication has 33 references indexed in Scilit:
- Investigation of Foscan® interactions with plasma proteinsBiochimica et Biophysica Acta (BBA) - General Subjects, 2005
- Inclusion of a Photosensitizer in Liposomes Formed by DMPC/Gemini Surfactant: Correlation between Physicochemical and Biological Features of the ComplexesJournal of Medicinal Chemistry, 2005
- pH-Dependent Modification of Lipophilicity of Porphyrin-type PhotosensitizersPhotochemistry and Photobiology, 2004
- Effects of Photodynamic Therapy on Tumor StromaUltrastructural Pathology, 2004
- Effects of Fluence Rate on Cell Survival and Photobleaching in Meta-Tetra-(hydroxyphenyl)chlorin–photosensitized Colo 26 Multicell Tumor Spheroids¶Photochemistry and Photobiology, 2001
- Fatty Acid Transfer from Liver and Intestinal Fatty Acid-binding Proteins to Membranes Occurs by Different MechanismsPublished by Elsevier BV ,1996
- Evaluation of a new photosensitizer, meso‐tetra‐hydroxyphenyl‐chlorin, for use in photodynamic therapy: A comparison of its photobiological properties with those of two other photosensitizersInternational Journal of Cancer, 1994
- Mechanism of the spontaneous transfer of phospholipids between bilayersBiochemistry, 1980
- Kinetics of phospholipid exchange between bilayer membranesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1977
- The Activated Complex in Chemical ReactionsThe Journal of Chemical Physics, 1935