Cloning and expression of the putative gene coding for GTP cyclohydrolase I from Escherichia coli

Abstract

The putative gene coding for GTP cyclohydrolase I of Escherichia coli was isolated from a λgt11 expression vectori library by using antibodies as a probe and has been subcloned on a 3.8 kb BamHI fragment in the plasmid vector pUC13. E. coli cells carrying the recombinant plasmid designated pCYH express 100-fold increased levels of the enzyme. The protein formed under the control of the plasmid appears electrophoretically and immunochemically identical with the wild type enzyme.