Nerve Growth Factor Expression after Plantar Incision in the Rat
- 1 July 2007
- journal article
- Published by Wolters Kluwer Health in Anesthesiology
- Vol. 107 (1), 128-135
- https://doi.org/10.1097/01.anes.0000267512.08619.bd
Abstract
Postoperative pain control remains a significant problem. Advances will proceed if we can further reveal the underlying mechanisms of incisional pain and its mediators. Previous studies have demonstrated that nerve growth factor (NGF) is released in incised tissue and contributes to hyperalgesia in incisional pain. The purpose of this study is to examine the expression of NGF in skin after planter incision. Adult Sprague-Dawley rats underwent incision at the plantar aspect of hind paw. The NGF messenger RNA (mRNA) was measured at various times after incision by polymerase chain reaction. NGF protein expression was detected by Western blot and immunohistochemistry in incisions. NGF mRNA increased from 2 to 4 h after incision and was the same as control by postoperative day 1. A large-molecular-weight form of NGF, approximately 75 kd, was found in normal skin. The large-molecular-weight NGF protein increased 4 h after incision and returned to baseline on postoperative day 7. The skin immediately adjacent to the incision had the greatest NGF expression. Immunohistochemical staining for NGF was present adjacent to the incision and localized in Schwann cells and axons. NGF mRNA is increased and a large-molecular-weight form of NGF protein is expressed in the region adjacent to the incision. NGF immunoreactivity is present in nerve bundles; both Schwann cells and axons are labeled. Immunoreactive NGF in axons is likely taken up into cut axons. This study suggests some common mechanisms for neuropathic and incisional pain.Keywords
This publication has 29 references indexed in Scilit:
- Lactate Concentrations in Incisions Indicate Ischemic-like Conditions May Contribute to Postoperative PainThe Journal of Pain, 2007
- Increased nerve growth factor after rat plantar incision contributes to guarding behavior and heat hyperalgesiaPain, 2005
- Changes in Tissue pH and Temperature after Incision Indicate Acidosis May Contribute to Postoperative PainAnesthesiology, 2004
- Effect of blockade of nerve growth factor and tumor necrosis factor on pain behaviors after plantar incisionThe Journal of Pain, 2004
- Molecular mechanisms of nociceptionNature, 2001
- The Effects of Peripheral Administration of a Novel Selective Antagonist for Prostaglandin E Receptor Subtype EP1, ONO-8711, in a Rat Model of Postoperative PainAnesthesia & Analgesia, 2001
- Intradermal recombinant human nerve growth factor induces pressure allodynia and lowered heat-pain threshold in humansNeurology, 1997
- Update of the NGF sagaJournal of the Neurological Sciences, 1995
- Nerve growth factor contributes to the generation of inflammatory sensory hypersensitivityNeuroscience, 1994
- The effect of systemically administered recombinant human nerve growth factor in healthy human subjectsAnnals of Neurology, 1994