Immobilization of urea cycle enzymes. I. Characterization of immobilized carbamoylphosphate synthetase and ornithine carbamoyltransferase
- 1 September 1977
- journal article
- research article
- Published by Wiley in Journal of Biomedical Materials Research
- Vol. 11 (5), 743-754
- https://doi.org/10.1002/jbm.820110509
Abstract
Carbamoylphosphate synthetase (EC 2.7.2.5) and ornithine carbamoyl-transferase (EC 2.1.3.3) extracted from frog liver were successfully immobilized on CNBr-activated Sepharose 4B. The immobilized preparation had a better stability towards heat. The apparent Michaelis constant values for N-acetyl-glutamate, ammonia, and ATP were not significantly changed by immobilization.This publication has 10 references indexed in Scilit:
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