Effect of cholesterol in membranes. Pulsed nuclear magnetic resonance measurements of lipid lateral diffusion
- 1 April 1981
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 20 (8), 2204-2207
- https://doi.org/10.1021/bi00511a020
Abstract
Lateral diffusion coefficients of lipids in a bilayer can be measured directly in a macroscopically aligned sample by use of a pulsed NMR method with pulsed magnetic field gradients. This technique was utilized to investigate the influence of cholesterol on the lipid diffusion of egg yolk lecithin, palmitoyloleoyllecithin and dioleoyllecithin. Cholesterol has a very small effect on the phospholipid diffusion. Cholesterol has a great influence on the molecular ordering in the bilayer and on the lipid phase structure. Cholesterol probably exerts its dominant effect on the lipid membrane stability.This publication has 17 references indexed in Scilit:
- Effect of cholesterol on the molecular motion in the hydrocarbon region of lecithin bilayers studied by nanosecond fluorescence techniquesBiochemistry, 1978
- Spectroscopic studies of specifically deuterium labeled membrane systems. Nuclear magnetic resonance investigation of the effects of cholesterol in model systemsBiochemistry, 1978
- Polymorphic phase behaviour of lipid mixtures as detected by 31P NMR. Evidence that cholesterol may destabilize bilayer structure in membrane systems containing phosphatidylethanolamineBiochimica et Biophysica Acta (BBA) - Biomembranes, 1978
- Influence of cholesterol on the polar region of phosphatidylcholine and phosphatidylethanolamine bilayersBiochemistry, 1978
- Phospholipid head-group conformations; intermolecular interactions and cholesterol effectsBiochemistry, 1977
- Glycerophospholipid synthesis: improved general method and new analogs containing photoactivable groups.Proceedings of the National Academy of Sciences, 1977
- Lateral diffusion in phospholipid multibilayers measured by fluorescence recovery after photobleachingBiochemistry, 1977
- Orientation and flexibility of the choline head group in phosphatidylcholine bilayersBiochimica et Biophysica Acta (BBA) - Biomembranes, 1977
- Lateral Diffusion in Planar Lipid BilayersScience, 1977
- Amphiphile diffusion in model membrane systems studied by pulsed NMRBiophysical Chemistry, 1977