The characterization of cDNA clones coding for wheat storage proteins
Open Access
- 1 January 1983
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 11 (10), 2961-2977
- https://doi.org/10.1093/nar/11.10.2961
Abstract
Poly (A)+ RNA isolated from the developing wheat endosperm var. Chinese Spring, has been used as template for the connstruction of a cDNA library. Within the library, clones have been identified by in vitro translation of hybrid-selected mRNA which encode α/β glidin related sequences and gliadin related sequences. The DNA sequence of one such clone has been determined and it shows homology with that of a clone encoding a barley stroge protein, B-hordein. The sequence includes a tendem DNA repeat which is discussed in relation to the generation of diversity within the gliadins.Keywords
This publication has 19 references indexed in Scilit:
- Cloning and sequence analysis reveal structural variation among related zein genes in maizeCell, 1982
- Wheat Storage ProteinsPlant Physiology, 1982
- Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencingJournal of Molecular Biology, 1980
- Structural gene identification and mapping by DNA-mRNA hybrid-arrested cell-free translation.Proceedings of the National Academy of Sciences, 1977
- Plasmid Detection and Sizing in Single Colony LysatesScience, 1977
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975
- Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresisBiochemistry, 1975
- Alkylation of horseradish peroxidase with lodoacetateArchives of Biochemistry and Biophysics, 1968
- Resolution of Multiple Ribonucleic Acid Species by Polyacrylamide Gel Electrophoresis*Biochemistry, 1967
- A membrane-filter technique for the detection of complementary DNABiochemical and Biophysical Research Communications, 1966