Isolation of Cellulose-Degrading Bacteria and Determination of Their Cellulolytic Potential
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Open Access
- 18 January 2012
- journal article
- research article
- Published by Hindawi Limited in International Journal of Microbiology
- Vol. 2012, 1-5
- https://doi.org/10.1155/2012/578925
Abstract
Eight isolates of cellulose-degrading bacteria (CDB) were isolated from four different invertebrates (termite, snail, caterpillar, and bookworm) by enriching the basal culture medium with filter paper as substrate for cellulose degradation. To indicate the cellulase activity of the organisms, diameter of clear zone around the colony and hydrolytic value on cellulose Congo Red agar media were measured. CDB 8 and CDB 10 exhibited the maximum zone of clearance around the colony with diameter of 45 and 50mm and with the hydrolytic value of 9 and 9.8, respectively. The enzyme assays for two enzymes, filter paper cellulase (FPC), and cellulase (endoglucanase), were examined by methods recommended by the International Union of Pure and Applied Chemistry (IUPAC). The extracellular cellulase activities ranged from 0.012 to 0.196IU/mL for FPC and 0.162 to 0.400IU/mL for endoglucanase assay. All the cultures were also further tested for their capacity to degrade filter paper by gravimetric method. The maximum filter paper degradation percentage was estimated to be 65.7 for CDB 8. Selected bacterial isolates CDB 2, 7, 8, and 10 were co-cultured with Saccharomyces cerevisiae for simultaneous saccharification and fermentation. Ethanol production was positively tested after five days of incubation with acidified potassium dichromate.Keywords
This publication has 28 references indexed in Scilit:
- Filter Paper Degradation by Bacteria Isolated From Local Termite GutResearch Journal of Microbiology, 2008
- Contrasts in Cellulolytic Activities of Gut Microorganisms Between the Wood Borer,Saperda vestita(Coleoptera: Cerambycidae), and the Bark Beetles,Ips piniandDendroctonus frontalis(Coleoptera: Curculionidae)Environmental Entomology, 2005
- Effect of Inoculating Flower Stalks and Vegetable Waste with Ligno-cellulolytic Microorganisms on the Composting ProcessJournal of Environmental Science and Health, Part B, 2004
- THE GUT BACTERIA OF INSECTS: Nonpathogenic InteractionsAnnual Review of Entomology, 2004
- Wood degradation, and cellulase and ligninase production, by Trametes and other wood-inhabiting basidiomycetes from indigenous forests of ZimbabweMycological Research, 1998
- Simultaneous saccharification and fermentation of steam-pretreated willowEnzyme and Microbial Technology, 1995
- Measurement of cellulase activitiesPure and Applied Chemistry, 1987
- Cellulases: Biosynthesis and applicationsEnzyme and Microbial Technology, 1980
- Use of Dinitrosalicylic Acid Reagent for Determination of Reducing SugarAnalytical Chemistry, 1959
- THE ANALYSIS OF MIXTURES OF ETHYL ALCOHOL, ETHYL ACETATE, ACETIC ACID AND WATERJournal of the American Chemical Society, 1928