A tripartite ssDNA mycovirus from a plant pathogenic fungus is infectious as cloned DNA and purified virions
Open Access
- 1 April 2020
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science Advances
- Vol. 6 (14), eaay9634
- https://doi.org/10.1126/sciadv.aay9634
Abstract
Here, we describe a tripartite circular single-stranded (ss) DNA mycovirus, named Fusarium graminearum gemytripvirus 1 (FgGMTV1). The genome of FgGMTV1 comprises three circular ssDNA segments (DNA-A, DNA-B, and DNA-C). Sequence alignments and phylogenetic analyses showed that FgGMTV1 is nested within the family Genomoviridae. We also constructed the first infectious DNA clones of a DNA mycovirus. Our results show that DNA-A and DNA-B are mutually interdependent for their replication and are associated with severely reduced colony growth and hypovirulence. DNA-C relies on DNA-A and DNA-B for replication and is necessary for the recovery of abnormal fungal phenotypes. DNA-C also enhances the accumulation of viral DNA in infected fungi and permits stable colonization and easy transmission via conidia. This is the first multipartite DNA virus isolated from a fungus. Our phylogenetic analyses also suggest that the multipartite genome of FgGMTV1 may have evolved from a monopartite genome of an ancient genomovirus.Funding Information
- The National Key R&D Program of China (2018YFD0200500)
This publication has 58 references indexed in Scilit:
- A field guide to eukaryotic circular single-stranded DNA viruses: insights gained from metagenomicsArchiv für die gesamte Virusforschung, 2012
- Molecular Characterization of Tomato Leaf Curl China Virus, Infecting Tomato Plants in China, and Functional Analyses of Its Associated BetasatelliteApplied and Environmental Microbiology, 2011
- Functional Analysis of a Novel Motif Conserved across Geminivirus Rep ProteinsJournal of Virology, 2011
- A geminivirus-related DNA mycovirus that confers hypovirulence to a plant pathogenic fungusProceedings of the National Academy of Sciences, 2010
- Evolution of geminiviruses and their satellitesFEBS Letters, 2009
- Geminiviruses: a tale of a plasmid becoming a virusBMC Evolutionary Biology, 2009
- Recent developments in the MAFFT multiple sequence alignment programBriefings in Bioinformatics, 2008
- Artificial Infection of Rosellinia necatrix with Purified Viral Particles of a Member of the Genus Mycoreovirus Reveals Its Uneven Distribution in Single ColoniesPhytopathology®, 2007
- Genome Sequence, Full-Length Infectious cDNA Clone, and Mapping of Viral Double-Stranded RNA Accumulation Determinant of Hypovirus CHV1-EP721Journal of Virology, 2007
- Hypovirulence of Chestnut Blight Fungus Conferred by an Infectious Viral cDNAScience, 1992