Origin of replication of pBR345 plasmid DNA.
- 1 December 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 74 (12), 5265-5269
- https://doi.org/10.1073/pnas.74.12.5265
Abstract
A small (.apprx. 1100 base pairs) ColE1-type plasmid, pBR345, was constructed from plasmid pMBI [of Escherichia coli] by a series of in vitro recombinant manipulations. Approximately 9% of the supercoiled pBR345 DNA obtained from cultures amplified with chloramphenicol appears to be replicative intermediates with replicating eye structures of uniform size. Results obtained from EM and biochemical analyses have enabled us to localize the origin of replication at the same position as that reported for ColE1. A sequence of 420 nucleotides surrounding this origin was determined. A comparison between this sequence and the one determined for the origin of replication of ColE1 is presented.This publication has 21 references indexed in Scilit:
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