Pyrene Mineralization by Mycobacterium sp. Strain KMS in a Barley Rhizosphere
- 1 September 2007
- journal article
- Published by Wiley in Journal of Environmental Quality
- Vol. 36 (5), 1260-1265
- https://doi.org/10.2134/jeq2007.0008
Abstract
To determine whether the soil Mycobacterium isolate KMS would mineralize pyrene under rhizosphere conditions, a microcosm system was established to collect radioactive carbon dioxide released from the labeled polycyclic aromatic hydrocarbon. Microcosms were designed as sealed, flow-through systems that allowed the growth of plants. Experiments were conducted to evaluate mineralization of 14C-labeled pyrene in a sand amended with the polycyclic aromatic hydrocarbons degrading Mycobacterium isolate KMS, barley plants, or barley plants with roots colonized by isolate KMS. Mineralization was quantified by collecting the 14CO2 produced from 14C-labeled pyrene at intervals during the 10-d incubation period. Roots and foliar tissues were examined for 14C incorporation. Mass balances for microcosms were determined through combustion of sand samples and collection and quantification of 14CO2 evolved from radiolabeled pyrene. No pyrene mineralization was observed in the sterile control systems. Greater release of 14CO2 was observed in the system with barley colonized by KMS than in microcosms containing just the bacterium inoculum or sterile barley plants. These findings suggest that phytostimulation of polycyclic aromatic hydrocarbons mineralization could be applied in remediation schemes.Keywords
Funding Information
- National Science Foundation (IBN‐0346539)
- Utah Agricultural Experiment Station
This publication has 43 references indexed in Scilit:
- Study of Biochemical Pathways and Enzymes Involved in Pyrene Degradation by Mycobacterium sp. Strain KMSApplied and Environmental Microbiology, 2006
- Analysis of Pseudomonas putida KT2440 Gene Expression in the Maize Rhizosphere: In Vivo Expression Technology Capture and Identification of Root-Activated PromotersJournal of Bacteriology, 2005
- Analysis of Pseudomonas putida KT2440 Gene Expression in the Maize Rhizosphere: In Vitro Expression Technology Capture and Identification of Root-Activated PromotersJournal of Bacteriology, 2005
- Mycobacterium pyrenivorans sp. nov., a novel polycyclic-aromatic-hydrocarbon-degrading speciesInternational Journal of Systematic and Evolutionary Microbiology, 2004
- Assessing the potential of using phytoremediation for pyrene-contaminated soilsRemediation Journal, 2004
- Molecular Cloning, Nucleotide Sequence, and Expression of Genes Encoding a Polycyclic Aromatic Ring Dioxygenase from Mycobacterium sp. Strain PYR-1Applied and Environmental Microbiology, 2001
- Field Evaluation of Mulberry Root Structure with Regard to PhytoremediationBioremediation Journal, 1999
- Phenological development in spring cereals: response to temperature and photoperiod under northern conditionsEuropean Journal of Agronomy, 1996
- Exposure to carcinogenic PAHs in the environmentEnvironmental Science & Technology, 1992
- ES&T Special Report: Priority pollutants: I-a perspective viewEnvironmental Science & Technology, 1979