Genetic Diversity WithinColletotrichum acutatum sensuSimmonds
Open Access
- 1 June 2001
- journal article
- Published by Scientific Societies in Phytopathology®
- Vol. 91 (6), 586-592
- https://doi.org/10.1094/phyto.2001.91.6.586
Abstract
Isolates of Colletotrichum acutatum from several hosts were characterized by various molecular methods in comparison with morphological identification. Species-specific primer analysis was reliable for grouping C. acutatum isolates to their designated species. Arbitrarily primed polymerase chain reaction and A+T-rich DNA analyses identified four subgroups within C. acutatum. Subgroup I contained U.S. isolates from almond, apple, peach, and pecan, subgroup II contained isolates from anemone, olive, and strawberry, subgroup III contained isolates from almond (Israel) and strawberry (Spain), and subgroup IV contained a single isolate from anemone (the Netherlands). Likewise, sequence analysis of the internal transcribed spacer (ITS) 2 region alone or the complete ITS (ITS 1–5.8S-ITS 2) region grouped the isolates into the same four subgroups. Percent similarity of the complete ITS region within each cluster ranged from 99.6 to 100.0, 99.8 to 100.0, and 98.6% among subgroups I, II, and III, respectively. DNA sequence analysis of the ITS 2 region alone or the entire ITS 1-2 region was more informative than that of the ITS 1 region, which could only group the isolates into two main clusters. The molecular methods employed for studying genetic variation in populations of C. acutatum determined that this species is diverse, indicating that isolates within populations of each subgroup are not host specific.Keywords
This publication has 26 references indexed in Scilit:
- Identification of Subpopulations ofColletotrichum acutatumand Epidemiology of Almond Anthracnose in CaliforniaPhytopathology®, 1999
- Molecular characterization of Colletotrichum strains derived from strawberryMycological Research, 1999
- Morphological and molecular analysis of Colletotrichum acutatum sensu latoMycological Research, 1999
- Characterization ofColletotrichumSpecies Responsible for Anthracnose Diseases of Various FruitsPlant Disease, 1998
- Characterization of Colletotrichum acutatum Isolates Causing Anthracnose of Almond and Peach in CaliforniaPhytopathology®, 1997
- Frequency ofColletotrichumSpecies Causing Bitter Rot of Apple in the Southeastern United StatesPlant Disease, 1996
- Nucleotide sequence of the rDNA spacer 1 enables identification of isolates of Colletotrichum as C. acutatumMycological Research, 1994
- Molecular Genotyping of Colletotrichum Species Based on Arbitrarily Primed PCR, A + T-Rich DNA, and Nuclear DNA AnalysesExperimental Mycology, 1993
- Species of Colletotrichum on Xanthium (asteraceae) with comments on some taxonomic and nomenclatural problems in ColletotrichumMycological Research, 1991
- A family of conserved repetitive DNA elements from the fungal plant pathogenGlomerella cingulata (Colletotrichum lindemuthianum)Experimental Mycology, 1991