Increased Detectability of Plasma HIV‐1 RNA after Introduction of a New Assay and Altered Specimen‐Processing Procedures
- 15 November 2008
- journal article
- Published by Oxford University Press (OUP) in Clinical Infectious Diseases
- Vol. 47 (10), 1354-1357
- https://doi.org/10.1086/592693
Abstract
After changes to assay and specimen-processing methods, plasma human immunodeficiency virus type 1 (HIV-1) RNA was frequently detectable in patients who previously had well-suppressed HIV-1 RNA levels. This artifact is attributable to shipping frozen plasma in primary plasma preparation tubes and is not caused by the HIV-1 RNA detection assay; it can be avoided by shipping plasma in a secondary tube.Keywords
This publication has 10 references indexed in Scilit:
- Elevation of viral load by PCR and use of plasma preparation tubes for quantification of human immunodeficiency virus type 1Journal of Microbiological Methods, 2007
- Solid organ transplantation: referral, management, and outcomes in HIV-infected patients.2006
- Increased levels of HIV RNA detected in samples with viral loads close to the detection limit collected in Plasma Preparation Tubes™ (PPT)Journal of Clinical Virology, 2006
- Transient Viremia in HIV-Infected Patients and Use of Plasma Preparation TubesClinical Infectious Diseases, 2005
- Discordance between Viral Loads Determined by Roche COBAS AMPLICOR Human Immunodeficiency Virus Type 1 Monitor (Version 1.5) Standard and Ultrasensitive Assays Caused by Freezing Patient Plasma in Centrifuged Becton-Dickinson Vacutainer Brand Plasma Preparation TubesJournal of Clinical Microbiology, 2005
- Platelets May Affect Detection and Quantitation of HIV RNA in Plasma Samples With Low Viral LoadsJAIDS Journal of Acquired Immune Deficiency Syndromes, 2004
- Revised recommendations for HIV screening of pregnant women.2001
- Effect of adherence to newly initiated antiretroviral therapy on plasma viral loadAIDS, 2001
- Full suppression of viral load is needed to achieve an optimal CD4 cell count response among patients on triple drug antiretroviral therapyAIDS, 2000
- Plasma Viral Load and CD4+ Lymphocytes as Prognostic Markers of HIV-1 InfectionAnnals of Internal Medicine, 1997