A Histochemical Method for the Demonstration of Diphosphopyridine Nucleotide Diaphorase
Open Access
- 25 January 1958
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 4 (1), 29-38
- https://doi.org/10.1083/jcb.4.1.29
Abstract
The present investigation concerning the histochemical demonstration of DPN diaphorase follows the development of a new reagent, Nitro-BT, which has already been used successfully for the cytochemical localization of the succinic dehydrogenase system. The most consistently favorable results were obtained with the lactate-lactic dehydrogenase system buffered at pH 7.4. Using sections of rat kidney and stomach, it was found that the intensity of stain was optimal after 15 minutes incubation at 37[degree]C, conducted aerobically. By appropriate variations in the substrate mixture it was possible to selectively demonstrate the histochemical distribution of certain DPN-linked dehydrogenases in addition to DPN diaphorase. This was made possible by the special distribution of some of these dehydrogenases which distinguished them from one another. Of the dehydrogenases studied the distribution pattern of [beta]-hydroxybutyric dehydrogenase was the most singular. In the gastric mucosa [beta]-hydroxybutyric dehydrogenase was restricted to the cells of the mucous lining epithelium and the gland necks; and in the kidney the enzyme was limited to the cells of the proximal convoluted tubule and thick limbs of Henle''s loop. In contrast, lactic dehydrogenase like DPN diaphorase was demonstrable in almost all cytologic elements of both the stomach and the kidney.Keywords
This publication has 14 references indexed in Scilit:
- HISTOCHEMICAL LOCALIZATION OF SPECIFIC OXIDATIVE ENZYMES III. EVALUATION STUDIES OF TETRAZOLIUM STAINING METHODS FOR DIPHOSPHOPYRIDINE NUCLEOTIDE DIAPHORASE, TRIPHOSPHOPYRIDINE NUCLEOTIDE DIAPHORASE AND THE SUCCINDEHYDROGENASE SYSTEMJournal of Histochemistry & Cytochemistry, 1956
- HISTOCHEMICAL LOCALIZATION OF SPECIFIC OXIDATIVE ENZYMES: II. LOCALIZATION OF DIPHOSPHOPYRIDINE NUCLEOTIDE AND TRIPHOSPHOPYRIDINE NUCLEOTIDE DIAPHORASES AND THE SUCCINDEHYDROGENASE SYSTEM IN THE KIDNEYJournal of Histochemistry & Cytochemistry, 1956
- HISTOCHEMICAL LOCALIZATION OF SPECIFIC OXIDATIVE ENZYMES. IV. SOLUBLE OXIDATION-REDUCTION DYES AS AIDS IN THE HISTOCHEMICAL LOCALIZATION OF OXIDATIVE ENZYMES WITH TETRAZOLIUM SALTSJournal of Histochemistry & Cytochemistry, 1956
- COMPARATIVE DISTRIBUTION OF SUCCINIC DEHYDROGENASE IN SIX MAMMALS AND MODIFICATION IN THE HISTOCHEMICAL TECHNICJournal of Histochemistry & Cytochemistry, 1953
- On the usefulness of tetrazolium salts as histochemical indicators of dehydrogenase activityThe Anatomical Record, 1952
- Differentiation of Minimus Type C. diphtheriae by Slow Fermentation of DextroseScience, 1951
- The specificity of diaphorase (coenzyme factor)Biochemical Journal, 1940
- Isolation and properties of a flavoprotein from heart muscle tissueBiochemical Journal, 1939
- On the catalytic function of heart flavoproteinBiochemical Journal, 1939
- Coenzyme factor—a new oxidation catalystBiochemical Journal, 1938