A putative nuclear receptor coactivator (TMF/ARA160) associates with hbrm/hSNF2α and BRG‐1/hSNF2β and localizes in the Golgi apparatus
- 14 May 2002
- journal article
- Published by Wiley in FEBS Letters
- Vol. 520 (1-3), 127-132
- https://doi.org/10.1016/s0014-5793(02)02803-x
Abstract
An ATP‐dependent chromatin remodeling factor, SNF/SWI complex, acts as a coactivator for numerous transcriptional factors. One of the best‐documented examples is nuclear receptors, although the molecular mechanism for this coactivation has not been sufficiently elucidated. Here we show that hbrm/hSNF2α and BRG‐1/hSNF2β, the ATPase subunits of the human SNF/SWI complexes, specifically associate in vitro and in vivo with TATA element modulatory factor (TMF)/ARA160, which has been described as a binding protein to and coactivator for the androgen receptor. This interaction requires highly conserved N‐terminal regions of hbrm/hSNF2α and BRG‐1/hSNF2β and a C‐terminal region of TMF/ARA160. Immunofluorescence and Western blot studies revealed that the TMF isoforms differentially localize in the Golgi apparatus and the nucleus.Keywords
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