Effect of Testosterone Propionate on Tissue Protein Synthesis in the Castrated Male Rat.

Abstract

Injection of testosterone propionate to castrated male rats stimulated protein synthesis at the level of the sex linked organs, skin, and in subcutaneously implanted polyvinyl sponge. Other tissues studied showed no effect of testosterone on rate of protein labeling after receiving 1-C14-glycine. Determinations were made 5, 10, 20, 40 and 60 hours after the injection of the tracer amino acid. The unique behavior of the perineal muscle in contrast to other skeletal muscles points to its unsuitability for myotrophic assay of anabolic hormones. The tissues where anabolic activity was stimulated also showed a concomitant increase of radioactivity in their non-protein fractions.