Topical Antimicrobial Toxicity
- 1 March 1985
- journal article
- research article
- Published by American Medical Association (AMA) in Archives of Surgery
- Vol. 120 (3), 267-270
- https://doi.org/10.1001/archsurg.1985.01390270007001
Abstract
• Three topical antibiotics and four antiseptics (1% povidone-iodine, 0.25% acetic acid, 3% hydrogen peroxide, and 0.5% sodium hypochlorite) were directly applied to cultured human fibroblasts to quantitatively assess their cytotoxicity. The four antiseptics were found to be cytotoxic; all of the cytotoxic agents except hydrogen peroxide were subsequently found to adversely affect wound healing in an animal model. Comparison of bactericidal and cytotoxic effects of serial dilutions of these four topical agents indicated the cellular toxicity of hydrogen peroxide and acetic acid exceeded their bactericidal potency. Bactericidal noncytotoxic dilutions of povidone-iodine and sodium hypochlorite were identified. These experiments provide evidence that 1% povidone-iodine, 3% hydrogen peroxide, 0.5% sodium hypochlorite, and 0.25% acetic acid are unsuitable for use in wound care. This sequence of experiments could be used to identify bactericidal, noncytotoxic agents prior to their clinical use. (Arch Surg1985;120:267-270)Keywords
This publication has 6 references indexed in Scilit:
- Povidone-iodine and Tensile Strength of Wounds in RatsJournal Of Trauma-Injury Infection and Critical Care, 1980
- Cultured human fibroblasts as a model for evaluation of potential in vivo toxicity of membrane damaging antibioticsChemico-Biological Interactions, 1980
- The bacteriology of primary wound sepsis in potentially contaminated abdominal operations: The effect of irrigation, povidone-iodine and cephaloridine on the sepsis rate assessed in a clinical trialBritish Journal of Surgery, 1978
- A study of the effect of povidone-iodine on wound healingPublished by Oxford University Press (OUP) ,1977
- Studies in management of the contaminated woundThe American Journal of Surgery, 1969
- A standard model for tensiometric studiesJournal of Surgical Research, 1965