Vasculogenesis in the day 6.5 to 9.5 mouse embryo
Open Access
- 1 March 2000
- journal article
- Published by American Society of Hematology in Blood
- Vol. 95 (5), 1671-1679
- https://doi.org/10.1182/blood.v95.5.1671.005k39_1671_1679
Abstract
The process of vasculogenesis was characterized in the 6.5- to 9.5-day mouse embryo and in allantoic culture by analysis of spatial and temporal expression patterns of the endothelial or hematopoietic lineage-associated proteins, TAL1, Flk1, platelet/endothelial cell adhision molecule (PECAM), CD34, VE-cadherin, and Tie2. The study establishes that: (1) TAL1 and Flk1 are coexpressed in isolated mesodermal cells that give rise to endothelial cells and thus can be defined as angioblasts; (2) hematopoietic cells of blood islands express TAL1, but not Flk1; (3) vasculogenesis in the embryo proper is initiated by mesoderm fated to give rise to the endocardium; (4) the maturation/morphogenesis of blood vessels can be defined in terms of a sequential pattern of expression in which TAL1 and Flk1 are expressed first followed by PECAM, CD34, VE-cadherin, and later Tie2; and (5) TAL1 expression is down-regulated in endothelial cells of mature vessels.Keywords
This publication has 49 references indexed in Scilit:
- The SCL gene specifies haemangioblast development from early mesodermThe EMBO Journal, 1998
- Embryonic angiogenesis: A reviewThe Science of Nature, 1996
- VasculogenesisAnnual Review of Cell and Developmental Biology, 1995
- Role of the Flt-1 receptor tyrosine kinase in regulating the assembly of vascular endotheliumNature, 1995
- Distinct roles of the receptor tyrosine kinases Tie-1 and Tie-2 in blood vessel formationNature, 1995
- Failure of blood-island formation and vasculogenesis in Flk-1-deficient miceNature, 1995
- Absence of blood formation in mice lacking the T-cell leukaemia oncoprotein tal-1/SCLNature, 1995
- High affinity VEGF binding and developmental expression suggest Flk-1 as a major regulator of vasculogenesis and angiogenesisCell, 1993
- A novel endothelial-specific membrane protein is a marker of cell-cell contacts.The Journal of cell biology, 1992
- Fluorescence Microscopy: Reduced Photobleaching of Rhodamine and Fluorescein Protein Conjugates by n -Propyl GallateScience, 1982